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35,000 units of Alkaline Phosphatase (AP) is ideal for labeling
proteins such as streptavidin and antibody. The AP labeled proteins can
be used in immunoblotting and immunocytochemistry diagnostic
applications, including ELISA or Western blotting.
AP activity is > 1,600 units/mg of protein (one unit equals the amount
of enzyme that will hydrolyze 1.0 micromole of p-nitrophenol phosphate
per minute at 25°C and pH 9.6) and is supplied as a liquid in 0.005 M
Tris, pH 7. 35 kU is ~20 mg of actual protein.
CAS Number: 9001-78-9
Alternative Name: Calf Intestinal Alkaline Phosphatase (CIAP) |
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