Cat. No.

CCM01

Product Overview

Stromal Cell-Dreived Factor 1α produced in E. coli has a molecular mass of approximately 8 KDa.

Description

SDF-1α, a member of the chemokine α subfamily, chemottracts with T-lymphocytes, monocytes, pro-and pre-B cells, but not neutrophils. It is highly conserved between species with one amino acid substitution between human and mouse. Recombinant stromal cell-dreived factor 1α is expressed in E. coli.

Purity

>95% as determined by HPLC.

Specific Activity

Measured by its ability to chemoattract cultured human macrovascular endothelial cells.

Storage Buffer

Liquid. In PBS Buffer.

Gene Information

Gene Name

CXCL12

Synonyms

PBSF; SCYB12; SDF-1a; SDF-1b; SDF1; SDF-1; SDF1A; SDF1B; TLSF; TLSF-a; TLSF-b; TPAR1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; Stromal cell-derived factor 1 precursor; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); stromal cell-derived factor 1; stromal cell-derived factor 1 delta; stromal cell-derived factor 1 gamma; stromal cell-derived factor 1a

GeneID

6387

mRNA Refseq

NM_199168.2

Protein Refseq

NP_954637.1

MIM

600835

UniProt ID

P48061

Chromosome Location

10q11.1

Pathway

Axon guidance, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration

Function

Chemokine activity, chemokine activity, growth factor activity, signal transducer activity

Cat. No.

CCM02

Product Overview

Recombinant Human MIP-4/CCL18 produced in E. coli is a single, non-glycosylated polypeptide chain containing 69 amino acids and having a molecular mass of 7.8 kDa.

Description

CCL18, is a novel CC chemokine that is highly homologous to MIP-1α (61% amino acid sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-γ. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0 -10.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuMIP-4/CCL18 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL18

Synonyms

CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18; CCL18_HUMAN; C-C motif chemokine 18 [Precursor]; Small-inducible cytokine A18;Macrophage inflammatory protein 4; ulmonary and activation-regulated chemokine;CC chemokine PARC;Alternative macrophage activation-associated CC chemokine 1; Dendritic cell chemokine 1.

GeneID

6362

mRNA Refseq

NM_002988

Protein Refseq

NP_002979

MIM

603757

UniProt ID

P55774

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction.

Function

chemokine activity

Cat. No.

CCM03

Product Overview

Recombinant Human MIP-5/CCL15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 92 amino acids and having a molecular mass of 10.1 kDa.

Description

CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1γ, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0 -10.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuMIP-5/CCL15 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL15

Synonyms

LKN1;NCC3;SY15;HCC-2;Lkn-1;MIP-5;NCC-3; SCYL3;MIP-1d;SCYA15;HMRP-2B;chemokine (C-C motif) ligand 15; CCL15_HUMAN; MIP5; C-C motif chemokine 15 [Precursor]; Small-inducible cytokine A15;Macrophage inflammatory protein 5;MIP-5;Chemokine CC-2; MIP-1delta; Chemokine CC-2;Leukotactin-1;Mrp-2b; small inducible cytokine subfamily A (Cys-Cys), member 15.

GeneID

6359

mRNA Refseq

NM_004167

Protein Refseq

NP_004158

MIM

601393

UniProt ID

Q16663

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction

Function

chemoattractant activity; chemokine activity; heparin binding; heparin binding; signal transducer activity.

Cat. No.

CCM04

Product Overview

Recombinant Human NAP-2/CXCL7 produced in E. coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa.

Description

Neutrophil Activating Peptide 2 (NAP-2) is proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR-2 and to chemoattract and activate neutrophils. Although CTAP-III, β-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. Recently, it has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human neutrophils using a concentration range of 1.0-10.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuNAP-2/CXCL7 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

PPBP

Synonyms

PBP; TC1; TC2; TGB; LDGF; MDGF; TGB1; B-TG1; CTAP3; CXCL7; NAP-2; SCYB7; THBGB; LA-PF4; THBGB1; Beta-TG; CTAPIII; NAP-2-L1; TGB1;SCYB7; CXCL7_HUMAN; Platelet basic protein [Precursor]; C-X-C motif chemokine 7;Small-inducible cytokine B7;Leukocyte-derived growth factor;LDGF;Macrophage-derived growth factor;MDGF.

GeneID

5473

mRNA Refseq

NM_002704

Protein Refseq

NP_002695

MIM

121010

UniProt ID

P02775

Chromosome Location

4q12-q13

Pathway

Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration; Hemostasis.

Function

chemokine activity;glucose transmembrane transporter activity;growth factor activity.

Cat. No.

CCM05

Product Overview

Recombinant Human Lymphotactin produced in E. coli is a single, non-glycosylated polypeptide chain containing 92 amino acids and having a molecular mass of 10.0 kDa.

Description

Lymphotactin is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The spleen shows the highest level of lymphotactin compared to peripheral leukocytes, lung, colon and small intestine. Lymphotactin is chemotactic towards lymphocytes but not towards monocytes or neutrophils.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH7.4, 150mM NaCl.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T cells using a concentration range of 10.0-100.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuXCL1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

XCL1

Synonyms

LTN; ATAC; LPTN; SCM1; SCM-1; SCYC1; SCM-1a; chemokine (C motif) ligand 1; lymphotactin; XCL1_HUMAN; Lymphotactin [Precursor]; C motif chemokine 1;Cytokine SCM-1;SCM-1-alpha;Small-inducible cytokine C1;XC chemokine ligand 1.

GeneID

6375

mRNA Refseq

NM_002995

Protein Refseq

NP_002986

MIM

600250

UniProt ID

P47992

Chromosome Location

1q23

Pathway

Cytokine-cytokine receptor interaction.

Function

chemokine activity

Cat. No.

CCM06

Product Overview

Recombinant Human Eotaxin-2/CCL24 produced in E. coli is a single, non-glycosylated polypeptide chain containing 78 amino acids and having a molecular mass of 8.8 kDa.

Description

Eotaxin, also named MPIF-2 and Ckβ6, is a novel CC chemokine recently identified. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 selectively chemoattracts cells expressing CCR3 including eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells. Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells. The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (92 a.a. residues for the murine homolog, without C-terminal truncation).

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Endotoxin

Less than 1EU/mg of rHuEotaxin-2/CCL24 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL24

Synonyms

Ckb-6; MPIF2; MPIF-2; SCYA24; chemokine (C-C motif) ligand 24; eotaxin-2;MPIF2; CCL24_HUMAN; C-C motif chemokine 24 [Precursor]; Small-inducible cytokine A24;Myeloid progenitor inhibitory factor 2; CK-beta-6;Eosinophil chemotactic protein 2;Eotaxin-2.

GeneID

6369

mRNA Refseq

NM_002991

Protein Refseq

NP_002982

MIM

602495

UniProt ID

O00175

Chromosome Location

7q11.23

Pathway

Cytokine-cytokine receptor interaction.

Function

chemokine activity

Cat. No.

CCM07

Product Overview

Recombinant Human HCC-4/CCL16 produced in E. coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 11.2 kDa.

Description

Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract total human monocytes using a concentration range of 10.0 -100.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuHCC-4/CCL16 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL16

Synonyms

LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16; MGC117051; CCL16_HUMAN; C-C motif chemokine 16 [Precursor]; Small-inducible cytokine A16;IL-10-inducible chemokine;Chemokine LEC;Liver-expressed chemokine;Monotactin-1;Chemokine CC-4;Lymphocyte and monocyte chemoattractant.

GeneID

6360

mRNA Refseq

NM_004590

Protein Refseq

NP_004581

MIM

601394

UniProt ID

O15467

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction.

Function

chemoattractant activity;chemokine activity.

Cat. No.

CCM08

Product Overview

Recombinant Human IP-10/CXCL10 produced in E. coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8.5 kDa.

Description

IP-10 was originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that IP-10 mRNA is also induced by LPS, IL-1β, TNF-α, IL-12 and viruses. Additional cell typesthat have been shown to express IP-10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts,astrocytes, and smooth muscle cells. IP-10 is also expressed in psoriatic and lepromatous lesions of skin. The mousehomologue of human IP-10, Crg-2, has been cloned andshown to share approximately 67% amino acid sequenceidentity with human IP-10.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2μm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 10.0-100.0 ng/ml.

Endotoxin

Less than 1EU/μg of rHuIP-10/CXCL10 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation isstable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL10

Synonyms

C7;IFI10;INP10;IP-10;crg-2; CXL10_HUMAN;mob-1;SCYB10;gIP-10;Gamma-IP10 ;10 kDa interferon-gamma-induced protein; C-X-C motif chemokine 10 precursor; Small-inducible cytokine B10; chemokine (C-X-C motif) ligand 10; gamma IP10; interferon (gamma)-induced cell line; protein 10 from; interferon-inducible cytokine IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine B10; small inducible cytokine subfamily B (Cys-X-Cys), member 10.

GeneID

3627

mRNA Refseq

NM_001565

Protein Refseq

NP_001556

MIM

147310

UniProt ID

P02778

Chromosome Location

4q21

Pathway

Cytokine-cytokine receptor interaction; Toll-like receptor signaling pathway.

Function

cAMP-dependent protein kinase regulator activity;chemokine activity

Cat. No.

CCM09

Product Overview

Recombinant Human I-TAC/CXCL11 produced in E. coli is a single, non-glycosylated polypeptide chain containing 73 amino acids and having a molecular mass of 8.3 kDa.

Description

CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence,which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-γ  and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T-cells, neutrophils or monocytes.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2μm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human IL-2 activated T-Lymphocytes using a concentration range of 0.1-10.0 ng/ml.

Endotoxin

Less than 1EU/μg of rHuI-TAC/CXCL11 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation isstable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL11

Synonyms

IP9; Beta-R1; H174; IP-9; b-R1; ITAC;I-TAC; SCYB11; SCYB9B; MGC102770; C-X-C motif chemokine 11 precursor;Human beta-R1 mRNA, partial cds; CXL11_HUMAN; chemokine (C-X-C motif) ligand 11;Interferon-gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; Small-inducible cytokine B11; chemokine (C-X-C motif) ligand 11; small inducible cytokine B11; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member.

GeneID

6373

mRNA Refseq

NM_005409

Protein Refseq

NP_005400

MIM

604852

UniProt ID

O14625

Chromosome Location

4q21.2

Pathway

Cytokine-cytokine receptor interaction;Toll-like receptor signaling pathway.

Function

chemokine activity

Cat. No.

CCM10

Product Overview

Recombinant Human MCP-2/CCL8 produced in E. coli is a single, non-glycosylated polypeptide chain containing 76 amino acids and having a molecular mass of 8.9 kDa.

Description

MCP-2 and MCP-3 are two recently identified monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the threeMCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Purity

>96% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0 -100.0 ng/ml.

Endotoxin

Less than 1EU/μg of rHuMCP-2/CCL8 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation isstable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL8

Synonyms

HC14;MCP2;MCP-2;SCYA8;SCYA10; C-C motif chemokine 8 precursor; Monocyte chemoattractant protein 2; Monocyte chemotactic protein2; Small-inducible cytokine A8; chemokine (C-C motif) ligand 8; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); CCL8_HUMAN.

GeneID

6355

mRNA Refseq

NM_005623

Protein Refseq

NP_005614

MIM

602283

UniProt ID

P80075

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction.

Function

chemokine activity;heparin binding;signal transducer activity.

Cat. No.

CCM11

Product Overview

Recombinant Human MIP-3 alpha/CCL20 produced in E. coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 8.0 kDa.

Description

MIP-3 alpha/CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. MIP-3 alpha is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2μm filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 10.0 -50.0 ng/ml.

Endotoxin

Less than 1EU/μg of rHuMIP-3a/CCL20 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL20

Synonyms

CKb4; LARC; exodus-1; MIP-3-alpha ; ST38; MIP3A; MIP-3a; SCYA20; Beta chemokine exodus-1; C-C motif chemokine 20 precursor; chemokine (C-C motif) ligand 20; CC chemokine LARC; CCL20_HUMAN;  Human mRNA for CC chemokine LARC precursor, complete cds; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; Small-inducible cytokine A20; chemokine (C-C motif) ligand 20; small inducible cytokine subfamily A (Cys-Cys), member 20.

GeneID

6364

mRNA Refseq

NM_001130046

Protein Refseq

NP_001123518

MIM

601960

UniProt ID

P78556

Chromosome Location

2q33-q37

Pathway

Cytokine-cytokine receptor interaction.

Function

chemokine activity.

Cat. No.

CCM12

Product Overview

Recombinant Human ENA-78/CXCL5 produced in E. coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.0 kDa.

Description

Epithelial cell-derived neutrophil-activating peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines that has the Glu-Leu-Arg (ELR) motif preceding the CXC motif. Similar to other ELR containing CXC chemokines, ENA-78 is a potent neutrophil chemoattractant and activator. Proteolysis of ENA-78 with cathepsin G and chymotrypsin have yielded N-terminally truncated variants with increased biological activities. ENA-70 and ENA-74 represent truncated recombinant ENA-78 variants missing 8 and 4 aa residues, respectively, from the N-terminus. Recombinant ENA-70 and ENA-74 have been shown to have increased potency in neutrophil chemotaxis and myeloperoxidase and elastase release assays.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2µm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Specific Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood neutrophils  using a concentration range of 5.0-50.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuENA-78/CXCL5 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL5

Synonyms

ENA-78; SCYB5; ENA-78(1-78); ENA78; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5; chemokine (C-X-C motif) ligand 5; epithelial-derived neutrophil activating protein 78; epithelial-derived neutrophil-activating peptide 78; neutrophil-activating protein 78; small inducible cytokine B5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); CXCL5_HUMAN; C-X-C motif chemokine 5 [Precursor].

GeneID

6374

mRNA Refseq

NM_002994

Protein Refseq

NP_002985

MIM

600324

UniProt ID

P42830

Chromosome Location

4q12-q13

Pathway

Cytokine-cytokine receptor interaction

Function

Chemokine activity