Cat. No.

CCM13

Product Overview

Recombinant Human Fractalkine/CX3CL1 produced in E. coli is a single, non-glycosylated polypeptide chain containing 76 amino acids and having a molecular mass of 8.5 kDa.

Description

Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human fractalkine cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human fractalkine can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble fractalkine. The soluble chemokine domain of human fractalkine was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse fractalkine was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Endotoxin

Less than 1EU/mg of rHuFractalkine/CX3CL1 as determined by LAL method.

Reconstitutionon

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CX3CL1

Synonyms

ABCD-3; C3Xkine; CXC3; CXC3C; NTN; NTT; SCYD1; fractalkine; neurotactin; A-152E5.2; C3Xkine; FKN; NTN; NTT; Neurotactin; fractalkine; neurotactin; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Small-inducible cytokine D1; chemokine (C-X3-C motif) ligand 1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; X3CL1_HUMAN; Fractalkine [Precursor].

GeneID

6376

mRNA Refseq

NM_002996

Protein Refseq

NP_002987

MIM

601880

UniProt ID

P78423

Chromosome Location

16q13

Pathway

Cytokine-cytokine receptor interaction

Function

chemokine activity.

Cat. No.

CCM14

Product Overview

Recombinant Human GRO-alpha/CXCL1 produced in E. coli is a single, non-glycosylated polypeptide chain containing 73 amino acids and having a molecular mass of 7.8 kDa.

Description

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Endotoxin

Less than 1EU/mg of rHuGRO-alpha/CXCL1 as determined by LAL method.

Reconstitutionon

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storagee

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL1

Synonyms

FSP; GRO; GRO1; GROA; GROa; MGSA; MGSA-a; NAP-3; SCYB1; MGSA alpha; C-X-C motif chemokine 1; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); Melanoma growth stimulatory activity; Neutrophil-activating protein 3; chemokine (C-X-C motif) ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); fibroblast secretory protein; melanoma growth stimulatory activity alpha; GROA_HUMAN; Growth-regulated alpha protein [Precursor]; GRO-alpha(1-73).

GeneID

2919

mRNA Refseq

NM_001511

Protein Refseq

NP_001502

MIM

155730

UniProt ID

P09341

Chromosome Location

4q21

Pathway

Cytokine-cytokine receptor interaction; Epithelial cell signaling in Helicobacter pylori infection

Function

chemokine activity; enzyme activator activity; growth factor activity

Cat. No.

CCM15

Product Overview

Recombinant Human GRO-beta/CXCL2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 73 amino acids and having a molecular mass of 7.9 kDa.

Description

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Specific Activityty

Fully biologically active when compared to standard. Determined by its ability to chemoattract hCXCR2 transfected 293 cells using a concentration range of 10.0-100.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuGRO-beta/CXCL2 as determined by LAL method.

Reconstitutionon

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storagee

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL2 chemokine (C-X-C motif) ligand 2

Synonyms

GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a; MIP2-alpha; MIP-2a; Gro-beta; C-X-C motif chemokine 2; GRO2 oncogene; Growth-regulated protein beta; MGSA beta; chemokine (C-X-C motif) ligand 2; melanoma growth stimulatory activity beta; MIP2A_HUMAN; Macrophage inflammatory protein 2-alpha [Precursor]; Gro-beta.

GeneID

2920

mRNA Refseq

NM_002089

Protein Refseq

NP_002080

MIM

139110

UniProt ID

P19875

Chromosome Location

4q21

Pathway

Cytokine-cytokine receptor interaction

Function

chemokine activity

Cat. No.

CCM16

Product Overview

Recombinant Human GRO-gamma/CXCL3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 73 amino acids and having a molecular mass of 7.9 kDa.

Description

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Specific Activityty

Fully biologically active when compared to standard. Determined by its ability to chemoattract hCXCR2 transfected 293 cells using a concentration range of 10.0-100.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuGRO-gamma/CXCL3 as determined by LAL method.

Reconstitutionon

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storagee

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL3 chemokine (C-X-C motif) ligand 3

Synonyms

GRO3; GROg; MIP2B; SCYB3; MIP-2b; CINC-2b; GRO-gamma; GRO-gamma(1-73); MIP2-beta; SCYB3; C-X-C motif chemokine 3; GRO3 oncogene; Growth-regulated protein gamma; MGSA gamma; chemokine (C-X-C motif) ligand 3; melanoma growth stimulatory activity gamma; Macrophage inflammatory protein 2-beta [Precursor]; MIP2B_HUMAN.

GeneID

2921

mRNA Refseq

NM_002090

Protein Refseq

NP_002081

MIM

139111

UniProt ID

P19876

Chromosome Location

4q21

Pathway

Cytokine-cytokine receptor interaction

Function

chemokine activity

Cat. No.

CCM17

Product Overview

Recombinant Human MIG/CXCL9 produced in E. coli is a single, non-glycosylated polypeptide chain containing 103 amino acids and having a molecular mass of 11.7 kDa.

Description

CXCL9, a member of the α subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-γ. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has recently been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

Purity

>97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.

Specific Activityty

Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood T-Lymphocytes using a concentration range of 10.0-100.0 ng/ml.

Endotoxin

Less than 1EU/mg of rHuMIG/CXCL9 as determined by LAL method.

Reconstitutionon

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storagee

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CXCL9 chemokine (C-X-C motif) ligand 9

Synonyms

CMK; MIG; Humig; SCYB9; crg-10; Gamma-interferon-induced monokine; Small-inducible cytokine B9; chemokine (C-X-C motif) ligand 9; monokine induced by gamma interferon; small inducible cytokine B9; CXCL9_HUMAN; C-X-C motif chemokine 9 [Precursor]; Gamma-interferon-induced monokine.

GeneID

4283

mRNA Refseq

NM_002416

Protein Refseq

NP_002407

MIM

601704

UniProt ID

Q07325

Chromosome Location

4q21

Pathway

Cytokine-cytokine receptor interaction;Toll-like receptor signaling pathway

Function

chemokine activity

Cat. No.

CCM18

Product Overview

Recombinant human MCP-4/CCL13 is 8.6 kDa, a single, non-glycosylated polypeptide chain containing 75 amino acids.

Description

CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.

Purity

>96% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl.

Specific Activityty

Fully biologically active when compared to standard. Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.

Endotoxin

Less than 1EU/μg of rHuMCP-4/CCL13 as determined by LAL method.

Reconstitutionon

It is recommended to reconstitute the lyophilized rHuMCP-4/CCL13 in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20^oC. Further dilutions should be made in appropriate buffered solutions.

Storagee

This lyophilized preparation is stable for several weeks at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC to -70^oC. Avoid repeated freeze/thaw cycles.

Gene Information

Gene Name

CCL13

Synonyms

CK-beta-10;CKb10; MCP-4; MCP4; MGC17134; NCC-1; NCC1; SCYA13; SCYL1; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; Small-inducible cytokine A 13;chemokine (C-C motif) ligand 13; new CC chemokine1; small inducible cytokine A 4; small inducible cytokine subfamily A (Cys-Cys), member 13; CCL13_HUMAN; C-C motif chemokine 13 [Precursor].

GeneID

6357

mRNA Refseq

NM_005408

Protein Refseq

NP_005399

MIM

601391

UniProt ID

Q99616

Chromosome Location

17q11.2

Pathway

Cytokine-cytokine receptor interaction.

Function

Chemokine activity; signal transducer activity.

Cat. No.

CCM19

Description

Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.A DNA sequence encoding the signal peptide from human CCL1 was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in HEK293 cell line.

Purity

> 90%, as determined by SDS-PAGE and visualized by silver stain.

Endotoxin

< 1.0 EU per 1 μg of the cytokine as determined by the LAL method.

Storage

Lyophilized samples are stable for up twelve months at -20℃ to -70℃.Upon reconstitution, this cytokine can be stored under sterile conditions at 2℃ - 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

CCL1 chemokine (C-C motif) ligand 1

Synonyms

P500; SISe; TCA3; I-309; SCYA1; small inducible cytokine A1; inflammatory cytokine I-309; T lymphocyte-secreted protein I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); chemokine (C-C motif) ligand 1;

GeneID

6346

mRNA Refseq

NM_002981

Protein Refseq

NP_002972

MIM

182281

Unitprot ID

P22362

Chromosome Location

17q12

Pathway

Cytokine-cytokine receptor interaction

Function

chemokine activity

Cat. No.

CCM20

Description

CXCL12 is strongly chemotactic for lymphocytes. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow and the formation of large blood vessels. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life.In adulthood CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPC) from the bone morrow through a CXCR4 dependent mechanism. It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression. A DNA sequence encoding the signal peptide from human chemokine (C-X-C motif) ligand 12 was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in HEK293 cell line.

Purity

> 90%, as determined by SDS-PAGE and visualized by silver stain.

Endotoxin

< 1.0 EU per 1 μg of the cytokine as determined by the LAL method.

Storage

Lyophilized samples are stable for up twelve months at -20℃ to -70℃.Upon reconstitution, this cytokine can be stored under sterile conditions at 2℃ - 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

CXCL12 chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)

Synonyms

PBSF; SDF1; SDF1A; SDF1B; TPAR1; hIRH; SDF-1; SCYB12; SDF-1a; SDF-1b; TLSF-a; Pre-B cell growth-stimulating factor;TLSF-b; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); stromal cell-derived factor 1; OTTHUMP00000019491; stromal cell-derived factor 1a; stromal cell-derived factor 1 delta; stromal cell-derived factor 1 gamma; C-X-C motif chemokine 12

GeneID

6387

mRNA Refseq

NM_000609

Protein Refseq

NP_000600

MIM

600835

Unitprot ID

P48061

Chromosome Location

10q11.1

Pathway

Axon guidance; Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration.

Function

chemokine activity; chemokine activity; growth factor activity;signal transducer activity

Cat. No.

CCM21

Production Description

SDF-1α and SDF-1β, members of the chemokine α subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1α and SDF-1β cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1α and SDF-1β are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1β and absent from SDF-1α.

Source

Escherichia coli.

Molecular Weight

8.5 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.

Quantity

2ug/10ug/1000μg

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human monocytes using a concentration range of 50.0-100.0 ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Endotoxin

Less than 1EU/μg of rMu SDF-1 beta /CXCL12 as determined by LAL method.

AA Sequence

KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRL KM

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Usage

This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Gene Information

Gene Name

Cxcl12 chemokine (C-X-C motif) ligand 12 [ Mus musculus ]

Synonyms

CXCL12; PBSF; SCYB12; SDF-1a; SDF-1b; SDF1; SDF1A; SDF1B; TLSF-a; TLSF-b; TPAR1; chemokine (C-X-C motif) ligand 12; Sdf1; TLSF; SDF-1; Sdf1a; Sdf1b; TPAR1; Scyb12; AI174028; PBSF/SDF-1; Cxcl12; stromal cell derived factor 1; stromal cell derived factor 1;OTTMUSP00000026112; OTTMUSP00000026113; OTTMUSP00000026114; pre-B-cell growth-stimulating factor; OTTHUMP00000019491; hIRH; C-X-C motif chemokine 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); stromal cell-derived factor 1; stromal cell-derived factor 1 delta; stromal cell-derived factor 1 gamma; stromal cell-derived factor 1a.

GeneID

20315

mRNA Refseq

NM_001012477

Protein Refseq

NP_001012495

UniProt ID

P40224

Chromosome Location

6 F1; 6 53.0 cM

Pathways

Axon guidance; Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration.

Function

Chemokine activity; cytokine activity; growth factor activity.

Cat. No.

CCM22

Product Overview

Recombinant Human CXCL13 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 87 amino acids and having a molecular mass of 10.3 kDa. The BCA-1 is purified by proprietary chromatographic techniques.

Description

BCA-1 is a CXC chemokine that is highly expressed in thesecondary lymphoid organs, such as follicles of the spleen, lymph nodes, and Peyer's patches. CXCL13 promotes the migration of B lymphocytes (compared to T cells and macrophages), by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). BCA1 therefore function in the homing of B lymphocytes to follicles. Human BCA-1 shares a 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA1.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Formulation

The BCA-1 protein was lyophilized from a concentrated (0.5 mg/ml) solution containing 20 mM PBS & 150 mM NaCl pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized CXCL13 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Specific Activity

Determined by its ability to chemoattract human B cells using a concentration range of 1.0-10.0 ng/ml.

Stability

Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

Gene Name

CXCL13 chemokine (C-X-C motif) ligand 13 [ Homo sapiens ]

Synonyms

BLC; BCA1; BCA-1; BLR1L; ANGIE; ANGIE2; SCYB13; CXCL13; B-cell chemoattractant; B-lymphocyte chemoattractant; B lymphocyte chemoattractant; B-cell-attracting chemokine 1; B cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); chemokine (C-X-C motif) ligand 13; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); CXC chemokine BLC; Small-inducible cytokine B13; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant).

GeneID

10563

mRNA Refseq

NM_006419

Protein Refseq

NP_006410

MIM

605149

UniProt ID

O43927

Chromosome Location

4q21

Pathways

Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration

Function

chemokine activity

Cat. No.

CCM23

Product Overview

Recombinant Human HCC-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 8411 Dalton. The HCC-1 is purified by proprietary chromatographic techniques.

Description

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is procesed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL13 activates monocytes, but does not induce their chemotaxis. Human CCL13 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Formulation

The CCL14 protein was lyophilized with 20 mM PBS pH 7.4 and 150 mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized HCC-1 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Specific Activity

The Biological activity is calculated by its ability to chemoattract Human monocytes at 5-20 ng/ml.

Stability

Lyophilized HCC1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

Gene Name

CCL14 chemokine (C-C motif) ligand 14 [ Homo sapiens ]

Synonyms

SCYA14; SY14; SCYL2; HCC-1; HCC-3; NCC-2; NCC2; CKb1; MCIF; CC-1; CC-3; CCL14;  FLJ16015; HCC-1(1-74); HCC-1/HCC-3; chemokine (C-C motif) ligand 14; chemokine CC-1; chemokine CC-3; Chemokine CC-1/CC-3; OTTHUMP00000176860; Small-inducible cytokine A14; small inducible cytokine subfamily A (Cys-Cys), member 14

GeneID

6358

mRNA Refseq

NM_004166

Protein Refseq

NP_004157

MIM

601392

UniProt ID

Q16627

Chromosome Location

17q11.2

Pathways

Cytokine-cytokine receptor interaction

Function

chemokine activity; protein binding; signal transducer activity

Cat. No.

CCM24

Product Overview

Recombinant Human CCL17 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 8 kDa. The TARC is purified by proprietary chromatographic techniques.

Description

Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Formulation

The protein was lyophilized from a concentrated (0.5 mg/ml) solution containing 20 mM PBS & 150 mM NaCl pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized CCL17 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Specific Activity

Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 1.0-10.0 ng/ml.

Stability

Lyophilized TARC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TARC should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

Gene Name

CCL17 chemokine (C-C motif) ligand 17 [ Homo sapiens ]

Synonyms

chemokine (C-C motif) ligand 17; TARC; ABCD-2; SCYA17; A-152E5.3; MGC138271; MGC138273; CCL17; small inducible cytokine A17; T cell-directed CC chemokine; thymus and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 17; CC chemokine TARC; OTTHUMP00000164673

GeneID

6361

mRNA Refseq

NM_002987

Protein Refseq

NP_002978

MIM

601520

UniProt ID

Q92583

Chromosome Location

16q13

Pathways

Cytokine-cytokine receptor interaction

Function

chemokine activity