Citrate buffer
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Citrate buffer pH6.0, 10X; Antigen Retriever Catalog number: AR-6544-02 100 ml AR-6544-05 1000 ml Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2-8oC, DO NOT FREEZE . Intended Use: 1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10 X, pH 6 antigen retriever solution. Preparation of working solution: Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2-8ºC Procedures:
Sometimes the buffer may show yellowish tinge, this is due to preservative and will not affect the function of the buffer.
Limitation: Tissue sections from brain or tissues in high concentration of lipids may get detached from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides. Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product. MSDS: Avoid skin and eye contact with all laboratory products. Use appropriate laboratory gear, laboratory coat , gloves and safety glasses. Do not ingest any laboratory products. This product is not approved for administration in human or animals. |
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