cytokeratin 10+13

Cytokeratin 10+13(CK 10+13) Mouse MAb anti-human

Catalog number: MM-1008-01    0.5 ml    Concentrated          IgG1    0.2 mg/ml

                              MM-1008-02    1.0 ml    Concentrated          IgG1     0.2 mg/ml   

Buffer: The concentrated antibodies are in supplied in PBS with 1% BSA, 0.05% azide, pH 7.4.

Description: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. With frozen sections, this antibody serves as a differentiation related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Recently Silveira et. al. (3) have reported that in tongue squamous cell carcinoma the expression of CK 10 was significantly correlated with the presence of metasis.

Intended Use: Immunohistochemistry (IHC) and Immunocytchemistry (ICC)

Storage:  2-8°C

Clone: CK  208                           Isotype: IgG2a

Epitope: Not determined

Molecular weight of antigen: CK 10 is 56.9 kDa and CK 13 is 53 kDa

Immunogen:. Cytokeratin extraction from human ectocervical epithelium

Species reactivity: Human, others –not-tested.

Cellular Localization: Cytoplasmic

Recommended positive control: Human tonsil or skin

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC) (frozen tissue sections ONLY or cell smears)

For IHC dilute conc. antibodies 1:50-1:100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.

The optimum dilution should be determined by the individual lab.

General References

1. Moll, R et al Cell, 31: 11-24, (1982)

2.      Ivanyi D,et. al; Cancer Res. 50: 5143-5152, 1990

3.      Silveira, EJ  et. al. Int. J Surg. Pathol. 15: 376-383, 2007

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the plumbing system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09