cytokeratin 8+18

Cytokeratin (CK 8+ 18) Mouse MAb anti-human

Catalog number:MM-1007-01    0.5 ml    Concentrated                        IgG1  0.2 mg/ml

                             MM-1007-02    1.0 ml    Concentrated                     .  IgG1  0.2 mg/ml   

                             MM-1007-04    7.0 ml    Prediluted for IHC                IgG1 ~5µg/ml

Buffer: The concentrated antibodies are supplied in PBS with 1% BSA,  0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Description: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.  This antibody recognizes all simple epithelia and glandular (thyroid, breast, GI tract, respiratory tract, urinary tract). Most squamous carcinomas and all adenocarcinomas are stained by this antibody and keratinizing squamous carcinomas are mostly negative.

Intended Use: Immunohistochemistry (IHC) and Immunocytchemistry (ICC)

Storage:  2-8°C

Clone:CK8+18 207                       Isotype: IgG1/ κ

Molecular weight of antigen: CK 8 is52.5 kDa and  CK 18 is 45 kDa

Immunogen:.Purified keratins

Species reactivity: Human, others –not-tested.

Cellular Localization: Cytoplasmic

Recommended positive control: Human breast, any GI tissue or skin

Application: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears.

For IHC dilute conc. antibodies 1:50-1:100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.

Prediluted antibody is ready to be used for IHC.

Antigen retriever like citrate enhances the staining of FFPE tissue sections) (Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT. The optimum dilution should be determined by the individual lab.

General References:

1. Moll, R et al Cell, 31: 11-24, (1982)

2.      Angus B et. al.J Path., 155: 71-75, 1988

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Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the plumbing system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09