Desmin, Polyclonal rabbit anti-human

Desmin, Polyclonal rabbit anti-human

Catalog number: RP-4023-01    0.5 ml       Concentrated:        IgG    3 mg/ml

                              RP-4023-02    1.0 ml       Concentrated          IgG    3 mg/ml

                              RP-4023-04    7.0 ml       Prediluted for IHC   IgG ~10 µg/ml

Description: Desmin is a type III intermediate filament found near the Z line in sarcomere. Desmin is only expressed in vertebrates, however homologous proteins are found in many organisms. It is a 52kD protein that is a subunit of intermediate filaments in skeletal muscle tissue, smooth muscle tissue, and cardiac muscle tissue. Antibodies to desmin are useful in identification of tumors of myogenic origin.

Buffer: Total immunoglobulin purified on Protein A column in10 mM PO4, 200 mM NaCl, pH 7.4, 1% BSA, 0.05% sodium azide.

Host: Rabbit

Reagent: Conc. antibody or prediluted for IHC

Storage:  2-8^oC.

Immunogen: Purified human Desmin

Molecular weight of antigen: 52 kDa

Positive control: Human muscle or sarcomas.

Cellular localization: Cytoplasmic

Species reactivity: Human, others not tested

Application: Immunohistochemistry (IHC) 1:50-1:200 using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears do require antigen retrieval (boiling tissue in 10 mM citrate buffer, pH 6.0 for 10 minutes, followed by cooling to RT for 10-15 minutes)

The optimum dilution should be determined by the individual lab

General References

1. Chang, TK and Smithson, WA. Arch. Pathol. Lab. Med. 113: 1343-1348, 1989.
2. Goebel, HH and Warlo, IAP, J Child Neurol., 15: 565-572, 2000.
3. Olivia, E, et. al. Am. J of Surg. Pathol. 19: 757-768, 1995.

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the pluming system and forms hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09