Description

The Polymerase gene from Thermus aquaticus (strain YT1) is cloned and expressed in E. coli, then highly purified to produce U-Taq DNA polymerase. It is used in the amplification and sequence testing of DNA through PCR. The quality of the U-Taq DNA polymerase has been tested by the Activity Test, SDS-Page, Endonuclease Test, etc.

Reaction Buffer (10X): 100 mM Tris-HCl, 400mM KCI, 15mM MgCl₂ , pH9.0

Dilution Buffer: 20mL Tris-HCl, 100mM KCI, 0.5 mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5 % IGEPAL CA-630(pH 8.0)

Storage Buffer: 20 mM Tris-HCI, 0.5 mM EDTA, 1mM DTT, 0,5% Tween 20, 0.5% IGEPAL CA-630(pH 8.0.). Store at -20℃.

Concentration: 5,000 units/mL

Involve the dNTPs mixture: The concentration of each dNTPs is 2.5 mM.

Description
Taq-red DNA Polymerase is an unique blend of U-Taq DNA Polymerase with an inert red dye. It offers the same performance as Taq DNA Polymerase . Several benefits are as following:

This red dye enables quick visual confirmation of enzyme addition and reaction mixing, which makes performance more convenient.
After PCR amplification, samples can be removed from the reaction and loaded directly onto  agarose gel without the addition of  loading buffer or tracking dye. The red dye, acting as a tracking dye, migrates between bromophenol blue and xylenecyanol at about the same rate as 400~500bp fragment.
The concentration of Taq-red is only 1unit/µl, so the enzyme can be transferred and added more accurately.
The enzyme generates PCR products with 3'-dA overhangs, suitable for T-A cloning.

Description
s-Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction, resulting in blunt-ended PCR products without 3'-dA overhangs. s-Pfu DNA Polymerase exhibits 3'→5' exonuclease (proofreading) activity that enables the polymerase to correct the mis-incorporation of nucleotide, and lacks 5'→3' exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter than 3kb.
The extension rate of s-Pfu DNA Polymerase is about 600bp/min in standard condition. The appropriate reaction temperature is 65℃~75℃, the working concentration of dNTPs is 100-300μM, the working concentration of Mg²⁺ is 2~3mM, and the optimal pH is 8.1~9.1. The amount of enzyme is 1~1.5unit for 20μl PCR reaction, while 2~3units for 50μl PCR reaction.

V-Taq DNA Polymerase

Description
Taq DNA Polymerase is the most common PCR enzyme for amplifying up to 10kb Lambda DNA templates and up to 3kb genomic templates. However, it is not able to amplify even longer DNA fragments. To overcome this constraint, variable PCR systems have been developed by various companies, which are containing Taq DNA Polymerase and thermostable DNA Polymerase with proofreading activity. V-Taq DNA Polymerase is the PCR System which are developed for amplifying long and complex fragments. It can amplify longer fragments up to 20kb from human genomic DNA, and up to 30kb from a Lambda DNA template). Moreover, it has improved amplification efficiency compared to Taq DNA Polymerase by improving enzyme activity. Therefore V-Taq DNA Polymerase is a more versatile enzyme blend in amplifying various templates including short and long DNA fragment or simple and complex DNA, either.

Description
The Easy-Do™ PCR PreMix is a pre-mixed preparation in a lyophilized format. The mix contains U-Taq DNA polymerase, reaction buffer, dNTPs, and tracking dye for efficient PCR amplification. For reaction set-up, all you have to do is to add templates, specific primers and water. After PCR amplification, samples can be loaded directly onto agarose gel without the addition of tracking dye. The PCR premix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination.
Storage: Store at -20℃ for one year.

Description
10mM dNTPs Mix is a ready-to-use solution of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 10mM each in sterile deionized water at pH7.5, whose purity is up to 99.5% (HPLC). It is free of RNase and DNase, and is suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation.
Storage: Store at -20℃.

Description
dNTPs in Separate Tube contains 4×0.4ml of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 100mM each in sterile deionized water at pH7.5, whose purity is up to 99.5% (HPLC). It is free of RNase and DNase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation.
Storage: Store at -20℃.

PCR enhancer can improve the efficiency of PCR amplification, increase the specificity of PCR products, and reduce non-specific and undesirable PCR products. The recommended amount is 1/10 of reaction volume.

GC-rich regions may form rigid secondary structure which makes difficult or impossible for the DNA polymerases to work under standard PCR conditions. The PCR optimizer can optimize PCR of problematic or GC-rich templates. The recommended amount is 1/5 of reaction volume.