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Product picture of DOTAP

DOTAP

A Well Established Transfection Reagent for Mammalian Cells

Product description

Biontex offers the well-established transfection reagent DOTAP as a ready-to-use solution. DOTAP is a liposome formulation of a monocationic lipid N-[1-(2,3-Dioleoyloxy)]-N,N,N-trimethylammonium propane methylsulfate in sterile water (concentration 1 mg/ml). Many experiments with this reagent (in vitro & in vivo) are described in the scientific literature.

It combines reliability and efficiency with low costs. If stored at 4°C it is stable for at least 12 months. Depending on the cell line, 1 ml of DOTAP is sufficient for 50-100 transfections on 35 mm dishes (6-well plates).

Structure of DOTAP
DOTAP
C43H83NO8S, MW: 774,21

DOTAP transfects DNA including yeast artificial chromosomes (YACs) both in the presence and absence of serum for transient and stable gene expression. DOTAP is additionally suitable for transfection of oligonucleotides, RNA, nucleotides, riboprotein complexes and proteins. In most cases serum containing transfection conditions result in higher transfection efficiency.

Highlights of DOTAP

  • Many experimental data available in the literature
  • No serum inhibition
  • In many cases sufficient transfection efficiency
  • Cytotoxicity below a concentration of 150µg/ml
  • Low price

 

Product Specifications
 
Application Transfection of nucleic acids into mammalian cells
Formulation Cationic lipids with colipids in water
Assays up to 1000 (24-well) or up to 300 (6-well) with 1 ml reagent
Sterility tested
Cell Culture tested
Shelf Life 1 year
Storage 4°C

Prices

Reagent Order No. Price
DOTAP 0,5 ml
Transfection Reagent for Mammalian Cells
T010-0.5 74 €
DOTAP 1 ml
Transfection Reagent for Mammalian Cells
T010-1.0 108 €
DOTAP 2 x 1 ml
Transfection Reagent for Mammalian Cells
T010-2.0 160 €
DOTAP 5 x 1 ml
Transfection Reagent for Mammalian Cells
T010-5.0 316 €
DOTAP 10 x 1 ml
Transfection Reagent for Mammalian Cells
T010-10.0 579 €
DOTAP 25 x 1 ml
Transfection Reagent for Mammalian Cells
T010-25.0 1304 €
DOTAP 50 x 1 ml
Transfection Reagent for Mammalian Cells
T010-50.0 2500 €

 

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Last modified: 05/13/09