EMA

Epithelial  Membrane  Antigen  (EMA), Mouse MAb anti- human

Catalog number: MM-1001-01,   0.5 ml     Concentrated          . IgG1    0.2 mg/ml

                              MM-1001-02    1.0 ml     Concentrated          . IgG1    0.2 mg/ml

                              MM-1001-04    7.0 ml     Prediluted for IHC   IgG1     4µg/ml

Buffer: The concentrated antibodies are supplied in PBS with 1% BSA,  0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Description: Epithelial membrane antigens are high molecular weight mucin-like glycoprotein. Antibodies to EMA have been shown to be useful as pan-epithelial markers for detection of early metastatic loci of carcinoma in the bone marrow or liver.

Host: Mouse

Intended Use: Immunohistochemistry (IHC) and Immunocytochemistry (ICC)

Reagent: Concentrated antibody

Storage:  2-8°C

Clone: EMA  201                                       Isotype: IgG1/κ

Epitope: DTRP

Molecular weight of antigen: 260-400 kDa

Immunogen: Human milk fat globule membranes

Species reactivity: Human, others –not-tested.

Cellular Localization: Cytoplasmic, cell membrane.

Recommended positive control: Breast CA, colon CA

Application: IHC, ICC (frozen or formalin –fixed paraffin-embedded (FFPE) tissue sections, cell smears.

For IHC dilute conc. antibodies 1:50-1:200, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.

Prediluted antibody is ready to be used for IHC.

The optimum dilution should be determined by individual lab.

General  References:

1. Gown, AM Human Pathology,19(1):119, 1988.
2. Russo, IH. In :Delellis RA, ed. Advances in Immunohistochemistry. New York: Raven Press, 431-75, 1988.

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the pluming system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09