For in vitro use only
Description:
FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’->3’ polymerization-dependent exonuclease replacement activity but lacks 3’-> 5’ exonuclease activity.
Applications:
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
Concentration:
5 U/µl
Storage and Dilution buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Shipping and Storage conditions:
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.
Reagents Provided:
- FIREPol® DNA Polymerase
- 10 x Reaction buffer B (Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
- 10 x Reaction buffer BD (Mg2+ and detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
- 10 x Solution S
Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
Related products:
|
+32
1658 9045
+32
1650 9045
