For in vitro use only
Description:
FIREPol®5 x Master Mixis a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).
Applications:
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
Reagent Composition:
- FIREPol® DNA polymerase
- 5 x Reaction Buffer B
0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 7.5 mM MgCl2 or 12.5 mM MgCl2
1 x PCR solution – 1.5 mM MgCl2 or 2.5 mM MgCl2
- 1 mM dNTPs of each
1 x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5 x Master Mix.
FIREPol® 5 x Master Mix Ready to Load
(7.5 mM MgCl2)
Cat. No. |
Pack size |
Price |
Conc. |
|
04-12-00115 |
1 ml |
70 € |
7.5 mM MgCl2 |
|
|
For in vitro use only
Description:
FIREPol® 5 x Master Mix Ready to Load is a premixed ready-to-use solution containing: all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
Applications:
- Suited for a wide range of PCR assays
- Primer extension
Reagent Composition:
- FIREPol® DNA polymerase
- 5 x Reaction Buffer B
0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 7.5 mM MgCl2 or 12.5 mM MgCl2
1 x PCR solution – 1.5 mM MgCl2 or 2.5 mM MgCl2
- 1 mM dNTPs of each
1 x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
- Blue dye
Migration equivalent to 3.5-4.5 kb DNA fragment
- Yellow dye
Migration rate in excess of primers in 1% agarose gel: <35-45 bp
- Compound that increases sample density for direct loading
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5 x Master Mix Ready to Load.
FIREPol® 5 x Master Mix
(12.5 mM MgCl2)
| Cat. No. |
Pack size |
Price |
Conc. |
|
04-11-00125 |
1 ml |
66 € |
12.5 mM MgCl2 |
|
|
For in vitro use only
Description:
FIREPol® 5 x Master Mixis a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).
Applications:
- Suited for a wide range of PCR assays
- Primer extension
- TA cloning
Reagent Composition:
- FIREPol® DNA polymerase
- 5 x Reaction Buffer B
0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 7.5 mM MgCl2 or 12.5 mM MgCl2
1 x PCR solution – 1.5 mM MgCl2 or 2.5 mM MgCl2
- 1 mM dNTPs of each
1 x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5 x Master Mix.
FIREPol® 5 x Master Mix Ready to Load
(12.5 mM MgCl2)
| Cat. No. |
Pack size |
Price |
Conc. |
|
04-12-00125 |
1 ml |
70 € |
12.5 mM MgCl2 |
|
|
For in vitro use only
Description:
FIREPol® 5 x Master Mix Ready to Load is a premixed ready-to-use solution containing: all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
Applications:
- Suited for a wide range of PCR assays
- Primer extension
Reagent Composition:
- FIREPol® DNA polymerase
- 5 x Reaction Buffer B
0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
- 7.5 mM MgCl2 or 12.5 mM MgCl2
1 x PCR solution – 1.5 mM MgCl2 or 2.5 mM MgCl2
- 1 mM dNTPs of each
1 x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
- Blue dye
Migration equivalent to 3.5-4.5 kb DNA fragment
- Yellow dye
Migration rate in excess of primers in 1% agarose gel: <35-45 bp
- Compound that increases sample density for direct loading
Shipping and Storage conditions:
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5 x Master Mix Ready to Load. |
|
|
Related products:
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+32
1658 9045
+32
1650 9045
