is a sensitive, stable and enviornmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen is far more sensitive than SYBR Safe (Figure 1). Unlike SYBR dyes, which are known to be unstable, GelGreen is very stable, both hydrolytically and thermally. GelGreen has a UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (Figure 2). Thus, GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader).
GelGreen can be used for either post gel staining or precast gel staining. However, GelGreen is not recommended for staining DNA or RNA in polyacrylamide gels due to the dye's slow diffusion rate in the relatively tight polyacrylamide gel matrix.
A series of safety tests have confirmed that GelGreenTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelGreen can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.
We offer GelGreenTM 10,000X solution in DMSO (cat#41004) and in water (cat#41005) for better safety. We also offer 10mL bulk pack size of GelGreenTM 10,000X solution in water.
- Safer than EB
Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
- Easy disposal
Passed environmental safety tests for direct disposal down the drain or in regular trash
Much more sensitive than EtBr and SYBR Safe
- Extremely stable
Available in water, stable at room temperature for long-term storage and microwavable
- Simple to use
Very simple procedures for either precast or post gel staining
- Perfectly compatible with a standard UV transilluminator or a gel reader with visible light excitation
GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 2)
- Perfectly compatible with downstream applications
Compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings.
Figure 1. Comparison of GelGreen, SYBR Green 1 and SYBR Safe in post gel staining using 1% agarose gel in TBE buffer. Two-fold serial dilutions of 1 kb Plus DNA Ladder from Invitrogen were loaded onto each gel in 4 lanes in the amounts of 200 ng, 100 ng and 50 ng, respectively, from left to right. Gels were imaged using 254-nm transillumination and photographed with a SYBR filter and Polaroid 667 black-and-white print films.
. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer