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METAFECTENE^®

The Highly Efficient RMA-based Transfection Reagent for Mammalian Cells

Product description

The high efficiency of METAFECTENE^® makes it a valuable tool for gene delivery or gene transfer experiments.

METAFECTENE^® is provided as a ready-to-use solution. Storage at 4°C for more than a year does not affect its transfection efficiency. It was also shown to be stable for weeks at 40°C. In contrast to most other transfection reagents METAFECTENE^® shows no serum inhibition, which makes it the reagent of choice for transfecting sensitive cell lines. Depending on the cell line, 1 ml METAFECTENE^® is sufficient for 50-200 transfections on 35 mm dishes (6-well plates) or 15 to 70 transfections on 60 mm dishes.

While METAFECTENE^® is suitable for a broad spectrum of applications, one of its best-known properties is its compatibility with high-throughput applications such as high-throughput screening (HTS). Other transfection methods either are not HTS compatible or need high amounts of DNA and reagent.

RMA-Technology

The unique properties of the reagent METAFECTENE^® are based on RMA-Technology ("Repulsive Membrane Acidolysis"), which ensures high transfection efficiency with low amounts of DNA and reagent. The combination, with its extremely simple instructions for use, delivers an outstanding price/performance ratio, an important consideration for both standard transfection procedures and especially for high-throughput assays.

The specifically designed molecular structure of METAFECTENE^® and METAFECTENE^® PRO provides for the following processes:

Extremely fast and easy procedure with METAFECTENE^®

 

Comparison of METAFECTENE^® with 5 other well known transfection reagents

Cells were transfected in triplicate with 0.1µg pND2Luc plasmid on 96 well plates. Luciferase activities were quantitated in a Promega luciferase assay by using a luminometer and are given as relative values of maximum transfection efficiencies (100%) for reagents with each cell line.

 

Cell Type	Description	Application Type	Results	Protocol
293	Human embryonic kidney	adherent
BHK	Baby hamster kidney	adherent
CHO	Chinese hamster ovary, epithelial	adherent
COS-7	Monkey kidney, fibroblastoid	adherent
CV-1	African green monkey kidney, fibroblastoid	adherent
HeLa-S3	Human cervix carcinoma, epithelial, subclone	adherent
Hep-G2	Human Liver hepatoblastoma, epithelial	adherent
NIH-3T3	Swiss mouse embryo, fibroblastoid	adherent
Vero	Monkey kidney	adherent

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Product Specifications
Application	Transfection of nucleic acids into mammalian cells
Formulation	Cationic lipids with colipids in water
Assays	up to 1000 (24-well) or up to 300 (6-well) with 1 ml reagent
Sterility	tested
Cell Culture	tested
Shelf Life	1 year
Storage	4°C

Prices

Reagent	Order No.	Price
METAFECTENE® 0,5 ml Transfection Reagent for Mammalian Cells	T020-0.5	111 €
METAFECTENE® 1 ml Transfection Reagent for Mammalian Cells	T020-1.0	173 €
METAFECTENE® 2 x 1 ml Transfection Reagent for Mammalian Cells	T020-2.0	303 €
METAFECTENE® 5 x 1 ml Transfection Reagent for Mammalian Cells	T020-5.0	648 €
METAFECTENE® 10 x 1 ml Transfection Reagent for Mammalian Cells	T020-10.0	1226 €
METAFECTENE® 25 x 1 ml Transfection Reagent for Mammalian Cells	T020-25.0	2890 €
METAFECTENE® 50 x 1 ml Transfection Reagent for Mammalian Cells	T020-50.0	5620 €