METAFECTENE® PRO
The Supreme Transfection Reagent for Mammalian Cells
METAFECTENE® PRO is the latest generation transfection reagent setting a new benchmark in mammalian cell transfection.
Its novel composition combines Biontex' RMA-Technology ("Repulsive Membrane Acidolysis") with a new Toxicity OPtimization module. (TOP-Technology)
TOP-Technology
Decreasing the toxic effects is one of the leading aims in the transfection technology. In-vitro applications normally benefit from lower toxicity but in-vivo applications must. The development of our TOP-Technology furthermore fits another great advantage: it moderately destabilizes the DNA-lipid-complex and potentiates the depletive access for the RMA!
Both techniques are excellently coordinated step-by-step. So this new product development consequently ensures the required release of genetic material in the cytoplasma and finally guarantees the availability of much more genetic material for uptake into the cell nucleus.
Highlights of METAFECTENE® PRO
Previous data support the fact that at least equivalent results can be achieved with METAFECTENE® PRO in comparison with METAFECTENE®, but multiple enhancement of efficiency can be obtained with moderate- or hard-to-transfect cell lines. In particular due to an optimum cytoplasmic release process and complete biodegradability a much higher level of transgene (protein) expression can be achieved generally compared to METAFECTENE®. Consequently METAFECTENE® PRO
is the ideal reagent for microscopy, viral production, biochemistry studies, protein production, etc.
Transfection efficiency of
METAFECTENE® PRO
METAFECTENE® as reference (100%), each optimized protocols
More results from independent scientists are listed here
Miscellaneous Applicability
METAFECTENE® PRO is adapted to:
- Co-transfection
- Reverse transfection
- Repetitive, multiple transfection
- All types of nucleic acids (siRNA, DNA, shRNA, mRNA)
- Pseudo-virus production
Easy and effective handling
METAFECTENE® PRO is provided as a ready-to-use solution. Experiment is rapidly handled in a 3-step procedure. It shows no serum inhibition, which makes it a reagent of choice for transfecting sensitive cell lines. Storage at - 20°C for one year is no problem in respect to transfection efficiency.
Depending on the cell line, 1ml of METAFECTENE® PRO is sufficient for 50-200 transfections on 35 mm dishes (6-well plates) or 15 to 70 transfections on 60 mm dishes.
| Product Specifications |
|
|
Application |
Transfection of nucleic acids into mammalian cells |
|
Formulation |
Cationic lipids with colipids in water |
|
Assays |
up to 1000 (24-well) or up to 300 (6-well) with 1 ml reagent |
|
Sterility |
tested |
|
Cell Culture |
tested |
|
Shelf Life |
1 year |
|
Storage |
≤ –15°C |
Prices
+32
1658 9045
+32
1650 9045
