µ-Transfection Kits

Cell culture, transfection and microscopy in a single slide

The µ–Transfection Kit VI combines the transfection efficiency of METAFECTENE^® µ (Biontex Laboratories GmbH) with µ-Slides  designed for cell microscopy. All components of the kit are coordinated for optimized and ultra-simple use.

METAFECTENE^® µ is a transfection reagent designed specifically for mammalian cell transfection using µ-Slide VI. The reagent combines low toxicity with outstanding transfection results and simple, rapid protocol.

When combined with µ–Slide VI and living cell microscopy, the reagent enables the processes following transfection to be examined.

The µ-Slide VI is designed for high-resolution microscopy and is suitable for experiments with living cells as well as for cell fixation and dyeing. The outstanding optical quality of the material enables fluorescence microscopy to be performed at high resolution and any wavelength required.

 

Contents of the µ-Transfection Kits
Each kit contains 15 µ-Slide VI, 100 ml transfection reagent and 10 ml 1x PBS

 

 

METAFECTENE^® µ

METAFECTENE^® µ and METAFECTENE^® µ FluoR labeled with rhodamine were designed specifically for mammalian cell transfection in µ-Slides VI. Thanks to the unique perfusion channel design of the µ-Slides VI, they offer outstanding optical conditions for high-end microscopy; however, they also pose significant challenges for the transfection reagent.

Unlike conventional cell culture vessels, the use of µ-slides is accompanied by diffusion effects and adsorptive loss of active transfection complexes and nutrients on surfaces. The composition of the transfection reagent METAFECTENE^® µ and simple standard protocol are aligned to the dimensions and composition of µ-Slide VI. METAFECTENE^® µ now enables outstanding transfection results to be obtained with µ-Slide VI, under excellent optical conditions for high-end microscopy which are impossible to obtain with conventional transfection reagents.

µ-Slides

µ-Slides are made of plastic of the maximum optical quality, which features ultra-low refraction and autofluorescence (both comparable with glass) and is resistant to a variety of chemicals including acetone or methanol. Because the plastic sheet base enables gas to be exchanged between the incubator atmosphere and the cell culture medium, the base must not be covered. µ-Slides should ideally be placed on an ibidi µ-Slide rack. Living or fixed cells may be observed directly on the µ-Slide under an inverse microscope. The base of the µ-Slides has a thickness of only 180µm and enables high-resolution microscopy to be carried out.