Peroxidase biotin
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Peroxidase~conjugated Biotin
Catalog number: AR-6638 1 mg 1 mg/ml
Description: The enzyme from horseradish is a glycoprotein, MW approximately 44 kDa, with hemin prosthetic group and bound Ca++. The RZ (Reinheitszahl) is the absorbance ratioA403/A275determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, and not enzymatic activity.
Presentation: The conjugate is supplied in one ml of 10 mM PO4, 200 mM NaCl, pH 7.4, BSA 10 mg/ml and 0.05% Proclin 300.
Application: ELISA1:10,000-1:50,000 (20-100 ng/ml); IHC: 1:200-1:1:1,000 (1-5 µg/ml); WB (ECL) 1:10,000-1:100,000 (10-100 ng/ml); WB (chromogen) 1:500-1:5,000 (0.1-2 µg/ml). The optimum dilution should be determined by the individual investigator.
Storage: 2-8oC
Dilution: This product can be diluted in our Universal antibody dilution buffer (cat. # 6562) or PBS with 0.5-1% BSA.
Precaution should be taken so that the buffer does not contain any sodium azide, which will destroy peroxidase.
Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product MSDS: Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals. |
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