peroxidase straptavidin
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Peroxidase~conjugated Streptavidin
Catalog number: AR-6633-01 1 mg 1 mg/ml Presentation: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0 One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed. Presentation: The conjugate is supplied in one ml of 10 mM PO4, 200 mM NaCl, pH 7.5, BSA 10 mg/ml and 0.05% Proclin 300. Application: ELISA1:10,000-1:50,000 (20-100 ng/ml); IHC: 1:200-1:1:1,000 (1-5 µg/ml); WB (ECL) 1:10,000-1:100,000 (10-100 ng/ml); WB (chromogen) 1:500-1:5,000 (0.1-2 µg/ml). The optimum dilution should be determined by the individual investigator. Storage: 2-8oC Dilution: This product can be diluted in our Universal antibody dilution buffer ( cat. # 6562)or PBS with 0.5-1% BSA.
Caution: Peroxidase reagents are destroyed by sodium azide; therefore it should be avoided in all buffers and reagents. Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product. MSDS: Use appropriate lab. gear, lab coat , gloves and safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals. |
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