phycoerythrin conjugated

Phycoerythrin, (R-PE) conjugated Streptavidin

Catalog number: AR-6635-01          1.0 ml

Description: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0.

Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band.

Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin;

Protease activity: 0.000018 units/mg

Isoelectric point: 7.0 +/- determined by Isoelectric focusing.

Phycoethyrin (R-PE) is a 240 kDa phycobilliprotein found in red, macrophytic algae (seaweed). R-PE can be used with Fluorescein (FITC) for double labeling in flow cytometry using argon laser, since both R-PE and FITC can be excited at 488 nm, and R-PE fluoresces at a longer wavelength (580 nm) than FITC (520 nm).

Phycoethyrin: R-PE purified from Poryhyra tenera; A565/A280 >5.0; A 1% 565 nm=82;

                        Amax = 490, 545 and 566 nm; E max = 580 nm

Presentation: Half mg of R-PE is conjugated with streptavidin, the unlabeled streptavidin is removed. Buffer 10 mM PO4, 150 mM NaCl,1% BSA and 0.05% sodium azide.

Application: Immunofluorescence (IF) 1: 50-1:200

                      Flow cytometry 1: 100-1:500 (not tested at our lab.

Storage: 2-8^oC.

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the plumbing system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2009 GENTAUR BVBA
Last modified: 05/13/09