phycoerythrin unconjugated

Phycoerythrin (R-PE), Unconjugated

Catalog number: AR-6636-01          1.0 mg           1 mg/ml

                              AR-6636-02          5.0 mg           5 mg/ml

Description: Phycoerythrin (R-PE) is a 240 kDa protein from the light-harvesting phycobilliprotein family, present in cyanobacteria, red algae and cryptomonads.Like all phycobilliprotein, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. In the phycoerythrin family, the phycobilins are: phycoerythrobilin, the typical phycoerythrin acceptor chromophore, and sometimes phycourobilin (marine organisms). Phycoerythrins are the phycobilliprotein which bind the highest number of phycobilins (up to six per alpha-beta subunit dimer).

Subunit structure:     (αβ)6γ

R-PE purified from Poryhyra tenera;

Absorption Maximum: 565 nm± 2 nm  (2 smaller  peaks are observed at 490 and 545 nm.

Emission Maximum:     580 nm

Purity:                            A565/A280 >5.0

Absorbance:                  A 1% 565 nm=82;

Presentation:. R-PE is supplied in 10 mM PO4, 150 mM NaCl buffer with 0.05% sodium azide.

Application: Conjugation of R-PE to Ig for FACS analysis. Before conjugation azide should be removed by dialysis or gel filtration column.

Storage: 2-8^oC.

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the plumbing system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09