PRO-Stain (I) Prestained protein marker

PRO-Stain (I) Prestained protein marker

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	PRO-STAIN^TM(I) Prestained Protein Marker for Broad Range is a mixture of purified proteins covalently coupled to a blue and violet dye that resolved to 9 bands between 9 and 198kDa when electrophoresed. The product contain high-concentration of PRO-STAIN^TM (I)Prestained Protein Marker for Broad Range, therefore very little amount is necessary of them. It designed for monitoring of protein separation during SDS-PAGE, verifying western blot transfer efficiency, perfect for CBB staining, silver staining and negative staining. Color remain the same even after blotting. PRO-STAIN^TM (I)Prestained Protein Marker is supplied in gel loading buffer and is ready-to-use.

The separation state of sample can be presumed during electrophoresis.
Contain high-concentraion of pre-stained protein, therefore very little amount is necessary of them.
Very good lot-to-lot consistency.
Perfect for CBB staining, silver staininng and negative staining.
Ready-to-use

- Store at -20ˇÉ, the quaranteed date is 2 yrs. Must be aliquot the protein marker in several tube, if more than stable.

Storage Buffer

3(W/V)ŁĄ SDS
10mM DTT
2mM EDTA-2Na
0.01(W/V)ŁĄ NaN3
10mM Tris-HCL (pH 6.8)
33(V/V)ŁĄ Glycerol

8-20ŁĄ SDS-PAGE Blot

Fig. 1. Apply 5 §ˇ on Mini-gel and electrophoresed for 1hr at 15% SDS-PAGE gel. After electrophoresed, transfer for 2hr at 80 volt.

PRO-STAIN^TM (I)Prestained Protein Marker(for Braod Range, Dual Color)

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Last modified: 05/13/09