qPCR

 
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QPCR

5 x HOT FIREPol^® Probe qPCR Mixes

Description:

5 x HOT FIREPol^® Probe qPCR Mix is an optimised ready-to-use solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and ROX dye according to system requirements. The user simply needs to add water, template, probe and primers.

Applications:

·         Detection and quantification of DNA and cDNA targets

·         Profiling gene expression

·         Microbial detection

·         Viral load determination

Shipping and Storage conditions:

Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® Probe qPCR Mix.

Performance Data:

HOT FIREPol^® Probe qPCR Mix performs very well even with the template concentration being as low as 312.5 pg.
Amplification of 72 bp fragment of human Albumin gene was performed on serial 4-fold dilutions of human genomic DNA (from 320 ng to 0.3 ng) in 3 replicate reactions of 20 µl. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.

Product name               Cat. No.                          Size                     Price

Kit contains:

5 x HOT FIREPol^® Probe qPCR Mix

5 x HOT FIREPol^® EvaGreen^® qPCR Mixes

Description:

HOT FIREPol^® EvaGreen^® qPCR Mix is an optimised ready-to-use solution for realtime quantitative PCR assays, incorporating EvaGreen^® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and ROX dye according to system requirements.

EvaGreen^® Dye:

EvaGreen^® is a DNA-binding dye with many features that make it a superior alternative to SYBR^® Green I for qPCR. Apart from having similar spectra, EvaGreen^® has three important features that set it apart from SYBR^® Green I: EvaGreen^® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen^® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR^® Green or FAM!

Applications:

·         Detection and quantification of DNA and cDNA targets

·         Profiling gene expression

·         High Resolution Melt (HRM) Analysis

·         Microbial detection

·         Viral load determination

Shipping and Storage conditions:

Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® EvaGreen^® qPCR Mix.

Performance Data:

Amplification of a 92 bp fragment from FBA1 gene was performed on serial fourfold dilutions of Saccharomyces cerevisiae genomic DNA. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System and ROX was used as a passive reference for normalization of the reporter signal.

Product name                           Cat. No.                         Size                        Price

Kit contains:

5 x HOT FIREPol^® EvaGreen^® qPCR Mix

 

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Last modified: 05/13/09