Red aqueuos counterstain

Red aqueous Counter Stain™

Catalog number: AR-6523-01    15 ml

                              AR-6523-02    100 ml

Description: This Red aqueous counter Stain is uniquely formulated for staining of protein when NBT/BCIP is used in Immunohistochemistry or in Situ hybridization. NBT/BCIP gives purple blue color; the chromogen is soluble in organic solvents and cannot be mounted with organic mounting medium.

Intended Use: Immunohistochemistry, in situ hybridization

Reagent: Ready to use counter stain.

Storage: Room temperature.

Procedure: In Situ hybridization, Immunohistochemistry, Immunocytochemistry.

1. After chromogen staining, wash slide 2-3X with distilled or deiononized water.

2. Apply Red aqueous counter stain for 3-5 minutes at RT.

3. Rinse slide to be mounted with Distilled or DEIONIZED water, touch the edges of slide on a paper towel to remove excess water and place the slides on a flat surface.

4. Apply 3-4 drops of Immuno In Situ mount mounting medium(AR-6507) directly on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Wait for 5 minutes. Excess mounting medium can be removed by touching the edges of slide against a paper towel.

5. Let stand at room temperature for about 1 to 2 hours, or the slides can be heated at 37º- 40^oC for 40-60 minutes. The slides are ready for visualization under a microscope, when 10X or 20X lenses are used.

There are two ways to look slide under oil immersion lens.

1. For oil immersion lens, a drop of oil can be placed directly on dried Immuno in situ mount mounting medium for visualization.

The oil can be removed by wiping with Kimwipes          OR

2. When the mounting medium has completely dried, apply 1-2 drops of any organic mounting medium on top of dried mounting medium, which has protected the specimen, cover slide with coverslip; the slide is ready for visualization.

Red aqueous counter stain is trade mark of ImmunoBioScience corp.

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09