S100 Protein Polyclonal rabbit anti-human

S100 Protein Polyclonal rabbit anti-human

Catalog number: RP-4014-01    0.5 ml       Concentrated          IgG    1 mg/ml

                              RP-4014-02    1.0 ml       Concentrated           IgG   1 mg/ml

                              RP-4014-04    7.0 ml       Prediluted for IHC   IgG ~5µg/ml

Description: S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH. Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells. This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.

Buffer: Total Ig fraction is supplied in10 mM PO4, 150 mM NaCl, pH 7.4, 1% BSA, 0.05% sodium azide.

Host: Rabbit

Intended Use Immunohistochemistry (IHC) and Immunocytchemistry (ICC)

Storage:  2-8°C

Immunogen: Molecular weight of antigen: ~22 kDa

Immunogen: .Purified S100 protein

Positive control: Melanoma or schwannoma

Cellular localization: Cytoplasmic

Species reactivity: Human, monkey, bovine, rat, mouse, others not tested.

Application: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. The optimum dilution should be determined by the individual lab.

THIS ANTIBODY SHOULD NOT BE DILUTED IN BUFFER WHICN CONTAINS DETERGENT.

General References

1.      Wosik, K et. al. J. Neurosci. 27: 9032-9042, 2007

2.      Joshi, MG et. al. Modern Pathol. 9: 57-62, 1996

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the pluming system and forms hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

---------------------------------------------------------------------------------------------------------------------------------

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2009 GENTAUR BVBA
Last modified: 05/13/09