The smartTM micro
BCA protein assay kit (for Micro Assay) is a
detergent-compatible bicinchoninic acid
formulation for the colorimetric detection and
quantitation of total protein. An adaptation of
the SMARTTM BCA Protein Assay Kit
(for Standard Assay), SMARTTM micro
BCA Protein Assay Kit (for Micro Assay) has been
optimized for use with dilute protein samples
(2-40 ug/ml).
The purple-colored reaction product of this
assay is formed by the chelation of two
molecules of BCA with one cuprous ion. This
water-soluble complex exhibits a strong
absorbance at 562 nm that is nearly linear with
increasing protein concentrations.
The macromolecular structure of protein, the
number of peptide bonds and the presence of four
particular amino acids(cystein, cystine,
tryptophan and tyrosine)are reported to be
responsible for color formation with BCA.
Studies with di-, tri- and tetrapeptides suggest
that the extent of color formation caused by
more than the mere sum of individual
colorproducing functional groups.
SMARTTM micro BCA Protein Assay Kit
(for Micro Assay) uses concentrated solutions
and a protocol that utilizes an extended
incubation time at an elevated temperature (60℃,
Test Tube Procedure only). The result is an
extremely sensitive colorimetric protein assay
in a microplate or test tube assay format.
- Colormetric method; read at
562 nm
- Compatible with most ionic and nonionic
detergents
- A very sensitive reagent for dilute protein
samples
- Linear working range for BSA: 0.5-20 ug/ml
- Less protein-protein variation that
dye-binding methods
- All kit reagents stable at room temperature
for 2 years
- Working solution is stable for 24 hours
- Convenient microplate format
* Solution A
100 ㎖
* Solution B
96 ㎖
* Solution C
4 ㎖
* BSA solution (128㎍/ml)
1 ㎖ x 10 ea
Storage at RT
(If either Solution A or Solution B precipitates
upon shipping in cold weather or during
long-term storage, dissolve precipitates by
gently warming and stirring solution. Discard
any kit reagent that shows discoloration or
evidence of contamination. )
Improved sensitivity & Linear working range
Fig. 1. Comparison of
sensitivity of protein assay
SMARTTM BCA Protein Assay Kit (for
Micro Assay) shows improved sensitivity higher
than competitor product as 15-20%.
The color response was estimated micro well
plate reader (37℃/120 minute incubation)
HIGH FIDELITY
Fig. 2. Typical color
response curves for BSA, Chymotrypsin and
Lysozyme using the Microplate Protocol.
Absorbance ratios (562 nm) for proteins relative
to BSA. The SMARTTM BCA Protein Assay
Kit (for Micro Assay) showed higher sensitivity
(slope) than Competitor. However, The
Protien-to-Protein variations showed similar
patters.
A; SMARTTM Micro BCA Protein
Assay Kit, B; Competitor A Product
+32
1658 9045
+32
1650 9045
Improved sensitivity & Linear working range
