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0032 (0)16 41 44 07

ChromaLink Biotin Protein Labeling Kit -- Protocol

Compound	*Sulfo ChromaLink Biotin 354* C38H49N8NaO13S2; Mol. Wt.: 912.96
Catalog #	B-1 007
Components	*Sulfo ChromaLink Biotin 354*
Purity	0.5 mg by optical density @ 354 nm, ε 29,000, in sodium phosphate buffer pH. 6.00
Application	Incorporation of biotin with a linker that possesses a chromaphore on amino-containing biomolecules and surfaces that allows quantification of biotin by spectroscopic means.
Comments	Store under inert atmosphere desiccated Not for internal or external use in humans.

Compound	Biotinylated Bovine IgG
Catalog #	P-1 002-001
Expiration date	3 months from date of receipt at 4^oC
Components	Biotinylated Bovine IgG @ 1.1 mg/ml (BCA assay) (1x Modification Buffer (pH 7.2) with 0.05% azide)
Concentration	1.1 mg/ml @ biotin molar substitution ratio 6.1 (A280/A354) biotin molar substitution ratio 5.9 (BCA/ A354)
Application	Biotinylated Bovine IgG protein standard
Comments	Store at 4^oC, do not freeze Not for internal or external use in humans.

Compound	Bovine IgG
Catalog #	P-1001-001
Components	Bovine IgG @ 0.95 mg/ml (BCA assay) (1x Modification Buffer (pH 7.4) with 0.05% azide)
Concentration	0.95 mg/ml
Application	Ready to biotinylate bovine IgG protein standard
Storage and Comments	Store at 4^oC, do not freeze Not for internal or external use in humans.

Product Name	Zeba^TM Spin Column, 0.5 ml
Catalog #	SoluLink Z-002-012 Pierce Chemical (Cat. # 89882)
Components	High performance polymeric resin contained in a polypropylene column w/lid
Purity	50% gel slurry
Application	Buffer exchange of proteins and desalting
Comments	Equilibrate in 1x Modification Buffer (pH 7.4) before use Not for internal or external use in humans
Storage Conditions	Store refrigerated 4-7^oC Do not freeze Not for internal or external use

Product Name	10X Modification Buffer
Catalog #	S-4003-002
Components	1.0 M Sodium Phosphate 1.5 M Sodium Chloride 0.05% Azide pH 7.4 (@ 1X concentration)
Comments	Use at 1x Modification Buffer Not for internal or external use in humans
Application	Concentrated buffer used at 1x concentration for optimized biotinylation of proteins with SulfoChromaLink Biotin
Storage Conditions	Store at room temperature Do not refrigerate

Product Name	10X PBS Buffer
Catalog #	S-4017-002
Components	1.0 M Sodium Phosphate 1.5 M Sodium Chloride 0.05% Azide pH 7.2 @ 1X concentration
Comments	Use at 1x PBS Buffer Not for internal or external use in humans
Storage Conditions	Store at room temperature

The products offered here are for research use only. Any commercial application will require a license from Solulink. The
Solulink Conjugation System is patented and has additional patents pending. Please contact Solulink for information regarding
licensing information.
No license is granted or implied to any patents to technologies for which the end user applies our products.

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Last modified: 05/13/09