Streptavidin
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Fluorescein (FITC) conjugated Streptavidin
Catalog number: AR-6634-01 1 mg 1 mg/ml
Description: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg Isoelectric point: 7.0 +/- determined by Isoelectric focusing. .One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed.
Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm
Fluorophore/Protein: A492nm/A280nm=~1 Presentation: The conjugated is one mg of streptavidin conjugated with Fluorescein isothiocyanate (FITC) in one ml of 10 mM PO4, 150 mM NaCl, pH 7.4, BSA 10 mg/ml and 0.05% sodium azide
Application: IF: 1:200-1:500 (2-5 µg/ml); Flow cytometry (FC): 1:1000-1:10,000 ( 0.1-1.0 µg/0.1 m l) This product is not tested is not tested in FC at our lab.. The optimum dilution should be determined by the individual investigator.
Storage: 2-8oC
Dilution: This product can be diluted in our Universal antibody dilution buffer (cat. no. AR-6562) or PBS with 0.5-1% BSA. Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product. MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the pluming system and forms hydrazoic acid in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals. |
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