Synaptophysin, Polyclonal rabbit anti-human

Synaptophysin, Polyclonal rabbit anti-human

Catalog number: RP-4008-01    0.5 ml    Concentrated               IgG 3 mg/ml;

                              RP-4008-02    1.0 ml     Concentrated              IgG 3mg/ml

                              RP-4008-04    7 .0 ml    Prediluted for IHC      IgG~10 µg/ml

Description: Synaptophysin is a synaptic vesicle glycoprotein with 4 transmembrane domain MW 38 kDa. This protein is present in neuroendocrine cells. It is also marker of endocrine tumors.

Buffer: Total immunoglobulin IgG purified by Protein A column in10 mM PO4, 150 mM NaCl, pH 7.4, 1% BSA, 0.05% sodium azide. Prediluted antibody in our Universal antibody dilution buffer (green color). (Cat. # AR-6562)

Host: Rabbit

Reagent: Conc. Antibody, Prediluted for IHC

Storage:  2-8^oC.

Epitope: C-terminus

Species reactivity: Human, others not tested.

Immunogen: A synthetic peptide from C-terminus of human Synaptophysin.

Positive control: Human pancreas or GI tumor

Cellular localization: Cytoplasmic

Application: Immunohistochemistry (IHC) for formalin-fixed paraffin embedded (FFPE) tissue sections and frozen sections. Dilute 1:100-1:200 using polymer or streptavidin ~biotin system for 30 mins at room temperature. The Prediluted antibody is ready to be used. This antibody requires antigen retriever (boiling FFPE tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling at  room temp. for 20 mins). The optimum dilution should be determined by the individual lab.

Specificity: This antibody reacts with Synaptophysin in neuroendocrine neoplasm of neural as well as epithelial type.

Reference: Dottorini, ME etal. Cancer: 77: 1556-1565 (1996).

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the pluming system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09