Thyroglobulin

Thyroglobulin (Tg) Mouse MAb anti-human

Catalog number: MM-1021-01    0.5 ml    Concentrated             IgG1    0.2 mg/ml

                              MM-1021-02    1.0 ml    Concentrated             IgG1    0.2 mg/ml  

                              MM-1021-04    7.0 ml    Prediluted for IHC     IgG1   5µg/ml

                              MM-1021-15    0.5 ml    FITC~conjugated      IgG1    0.2 mg/ml

                              MM-1021-16    1.0 ml    FITC~conjugated      IgG1     0.2 mg/ml

Buffer The antibodies are supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Description: Thyroglobulin (Tg) is a 660 kDa dimeric protein produced by and used by entirely within thyroid gland. Tg is used by the thyroid gland to produce the thyroid hormone thyroxine (T4) and triiodothyronine (T3). Tg is produced by the thyroid epithelial cells, called thyrocytes, which form spherical follicles. Tg is stored in the follicle lumen. Tg levels in the blood can be used as markers for certain kind of thyroid cancer. Tg levels are also elevated in Graves disease. Antibody to Tg is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of Tg in metastatic lesion establishes thyroid origin of tumor.

Intended Use: Immunohistochemistry (IHC) and Immunocytchemistry (ICC)

Immunofluorescence (IF), Flow cytometry (not tested at our lab).

Storage:  2-8°C

Clone: Tg 221                                 Isotype: IgG1/κ

Epitope: not known

Molecular weight of antigen:330 kDa

Immunogen: .Purified human Thyroglobulin

Species reactivity: Human, others not tested.

Cellular Localization: Cytoplasmic

Recommended positive control: Human thyroid

Application: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears.

For IHC dilute conc. antibodies 1:50-1:100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.

Prediluted antibody is ready to be used for IHC.

IF, FITC~conjugated antibodies 10-20 µg/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.

Flow: FITC~conjugated antibodies, 0.2-1.0 µg/0.1 ml (1:200-1:1,000) (Not tested in our lab).

The optimum dilution should be determined by the individual lab.

General References

1.       Ossendorp, FA et. al. J. Immunol. Methods. 120: 191-200, 1989

2.       Ossendorp, FA, et.al. 127: 419-430, 1990

Limitation and warranty: Our warranty is limited to the actual price paid for the product. We are not liable for any property damage, personnel injury, time, effort or economic loss due to our product.

MSDS: This product contains 0.05 % sodium azide as a preservative, appropriate care should be taken in handling. National Institute of Occupational Safety and Health has warning that sodium azide can react with lead, copper, brass or solder in the plumbing system and forms  hydrazoic acid  in acidic condition. Discard with copious amount of water. Avoid skin and eye contact with all laboratory products. Use appropriate lab. gear, lab coat , gloves and  safety glasses. Do not ingest any lab. products. This product is not approved for administration in human or animals.

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Last modified: 05/13/09